Image data can be obtained in a number of ways: hard copy photographs can be scanned with a flatbed scanner, color slides or black & white or color negatives can be scanned using a slide scanner or images can be input directly to the computer via a video system or digital camera attached to the microscope. There are differences between the images obtained from a video system and those from scanners. The scanner lets you set the resolution of the acquired images directly; the video does not. The quality of images from slides or negatives acquired from a scanner is dependent on the quality of the scanner used. Images should be acquired at the resolution and the size (height, width) (given in %) needed for the final output. With video, you need to have a good high resolution video system (with 750 horizontal lines) to get good images. In our lab, we occasionally use various scanners for images but most of our images come from video systems and video imaging is what this demonstration will focus on.